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Recombinant Hrv 3c Protease (His-tag) for Protein Purification

China Recombinant Hrv 3c Protease (His-tag) for Protein Purification, Find details about China Protein Purification Reagent, Protease His-Tag from Recombinant Hrv 3c Protease (His-tag) for Protein Purification

Model NO.
G3410-1000U
Habit Appellation
Fine Chemicals
Application
Industry, Scientific Research
Property
Enzyme & Coenzyme
Trademark
Servicebio
Specification
5000U
Origin
China
Model NO.
G3410-1000U
Habit Appellation
Fine Chemicals
Application
Industry, Scientific Research
Property
Enzyme & Coenzyme
Trademark
Servicebio
Specification
5000U
Origin
China
Product introduction
Recombinant HRV 3C Protease (Human Rhinovirus 3C Protease,Prescission, Human Rhinovirus type 14 3C Protease, a commonly used cysteine Protease, is a recombinant expression of His-tagged Human Rhinovirus type 14 3C Protease in Escherichia coli.It can specifically recognize the sequence L-E-V-L-F-Q-G-P (Leu-Glu-Val-Leu-Phe-Gly-Gly-Pro) or the core pentapeptide sequence L-F-Q-G-P (Leu-Phe-Gly-Gly-Pro) at low temperature.The enzyme digestion was performed between Q (Gln) and G-P (Gly-Pro) amino acid residues.The enzyme had strong specificity and high activity, and it still had high activity even at 4ºC.
Application: It is often used to remove MBP, GST, HIS or other tagged proteins from fusion proteins.The protease itself carries the HIS label and binds to the nickel column.
Source: Human Rhinovirus type 14 3C Protease, recombinant expression of Escherichia coli, containing His label.
Definition of enzyme activity: 100 μg of substrate (protein containing HRV 3C Protease digestion site) can be cut more than 90% of the required enzyme activity unit within 16h under the condition of 4ºC, pH 7.5.
Inactivation or inhibition: 100mM ZnCl2, 4mM AEBSF and 100μm Chymostatin all inhibit the activity of HRV 3C Protease.
Purity and concentration: SDS-PAGE purity ≥95%, 2 U/μL.
Recombinant HRV 3C Protease Storage solution:50 mM Tris-HCl,150 mM NaCl,1 mM EDTA,1 mM DTT,50% (v/v) glycerol,pH 8.0.
10×HRV 3C Buffer:500 mM Tris-HCl,1.5 M NaCl,10 mM EDTA,10 mM DTT,pH 7.5.
Storage and transportation:
Transport with wet ice; store at -20ºC, valid for 12 months.

Specification
Component NumberComponentG3410-1000U
G3410-1Recombinant HRV 3C Protease (His-tag) (2 U/μL)500 μL
G3410-210×HRV 3C Buffer3×1 mL
G3410-3Control Protein100 μL
Manual1
Operating steps
1. Protenase digestion system recommendation:
ComponentVolume
10×HRV 3C Buffer5 μL
Interest protein200 μg
Recombinant HRV 3C Protease (His-tag) (2U/μL)1 μL
ddH2OTo 50 μL
2. The reaction system was placed at 4ºC for overnight reaction or 16h;
3. 10μL sample of digestion product was taken for SDS-PAGE detection to evaluate the amount of digestion and reaction conditions.

Notes:
1. Enzyme products should be placed in an ice box or ice bath, and stored at -20ºC immediately after use. Separate storage is recommended.
2. For your safety and health, please wear a lab coat and disposable gloves to operate.

Examples of enzymatic digestion applications:
Recombinant Hrv 3c Protease (His-tag) for Protein Purification
 Figure 1: Effect of HRV 3C Protease Digestion Control Proteins (A+B fusion protein containing HRV 3C Protease Digestion Site) (Digestion condition: 30ºC, pH 7.5, Enzyme digestion for 0min, 10min, 20min, 30min, 1h, 2h, respectively, corresponding Lane Lane: 1-6, the mass ratio of substrate to enzyme added in the digestion system was 10:1).
Recombinant Hrv 3c Protease (His-tag) for Protein PurificationRecombinant Hrv 3c Protease (His-tag) for Protein PurificationRecombinant Hrv 3c Protease (His-tag) for Protein PurificationRecombinant Hrv 3c Protease (His-tag) for Protein PurificationRecombinant Hrv 3c Protease (His-tag) for Protein Purification

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