Hipure Circulating DNA Kit (Vacuum Protocol)

China Hipure Circulating DNA Kit (Vacuum Protocol), Find details about China Rapid Isolation of High-Quality Viral DNA /Rna, Chemistry Laboratory Reagent Magnetic Beads from Hipure Circulating DNA Kit (Vacuum Protocol)

Basic Info.

Model NO.

D3182

Color

Colorless

Appearance

Plate,Liquid,Powder

Type

for Lab

Trademark

Magen Biotech

Specification

50 preps/kit

Origin

China

HS Code

90279000

Model NO.

D3182

Color

Colorless

Appearance

Plate,Liquid,Powder

Type

for Lab

Trademark

Magen Biotech

Specification

50 preps/kit

Origin

China

HS Code

90279000

Product Desciption

Product Name Hipure Circulating DNA MIDI Kit B Free

Product specifications 50 Preps/Kit

Intended Use
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp(DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.The extracted products can be used for clinical in vitro detection.

Main Composition:

Cat.No	IVD3182	Contents
Buffer ACL	250 ml	Glycerol/Tris/CaCl2
Buffer ACB*	300 ml	Tris/EDTA/Guanidine Salt
Buffer DCW1*	22 ml	Tris/EDTA/Guanidine Salt
Buffer DCW2*	10 ml	Tris/Nacl
Proteinase K	540 mg	10mm Tris,pH8.0
Protease Dissolve Buffer	30 ml	Glycerol/Tris/CaCl2
Carrier RNA	110 µg	Poly A
Nuclease Free Water	20 ml	Water
HiPure CFDNA Mini Columns	50	Silicon Column
2 ml Collection Tubes	100	PP Column
Extender Tube	50	PP Column
Vac-Connector	50	PS Column

Storage conditions and Validity
This kit is shipped and stored at room temperature and is valid for 12 months.

Preparation before Use

1. Add 200 mIsopropanol to the bottle of Buffer ACB, and store at room temperature.

2. Add 28 ml absolute ethanol to the bottle of Buffer DCW1, and store at room temperature.

3. Add 40 ml absolute ethanol to the bottle of Buffer DCW2, and store at room temperature.

4.Add 27 ml Protease Dissolve Buffer to the bottle of proteinase K,store at -20ºC~8ºC after dissolve.

5.Add 0.55ml Nuclease Free Water to the bottle of carrier RNA, store at -20ºC after dissolve.

Reagent volume follow the table

Sample volumes	1ml	2ml	3ml	4ml	5ml
Proteinase K	100µl	200µl	300µl	400µl	500µl
Buffer ACL	0.8ml	1.6ml	2.4ml	3.2ml	4.0ml
Carrier RNA	5µl	5µl	5µl	5µl	5µl
Buffer ACB	1.8ml	3.6ml	5.4ml	7.2ml	9ml
Buffer DCW1	750ul	750ul	750ul	750ul	750ul
Buffer DCW2	750ul	750ul	750ul	750ul	750ul
100% Ethanol	750ul	750ul	750ul	750ul	750ul

Human plasma DNA extraction comparison between Qiagen with Magen

Hipure Circulating DNA Kit (Vacuum Protocol)

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Hipure Circulating DNA Kit (Vacuum Protocol)