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CE, ISO9001 Approved Mtor (S2442) Polyclonal Antibody

China CE, ISO9001 Approved Mtor (S2442) Polyclonal Antibody, Find details about China Protein, MEK from CE, ISO9001 Approved Mtor (S2442) Polyclonal Antibody

Model NO.
BS3611
Certification
CE, ISO9001
Host
Rabbit/Mouse
Reactivity
Human, Mouse, Rat
Trademark
Bioworld
Transport Package
Refrigerated Transport
Specification
100ug/1mg
Origin
China
HS Code
300219000
Model NO.
BS3611
Certification
CE, ISO9001
Host
Rabbit/Mouse
Reactivity
Human, Mouse, Rat
Trademark
Bioworld
Transport Package
Refrigerated Transport
Specification
100ug/1mg
Origin
China
HS Code
300219000
Catalog No.BS3611
Product NamemTOR (S2442) polyclonal antibody
ApplicationsWB IHC IF
Alternative NameSerine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamy
Swiss-ProtP42345
HostRabbit
ReactivityHuman,Mouse,Rat
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
ProductRabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
SpecificitymTOR (S2442) polyclonal antibody detects endogenous levels of mTOR protein.
BiowMW~ 289 kDa
NoteFor research use only, not for use in diagnostic procedure.
ImmunogenSynthetic peptide, corresponding to amino acids 2430-2480 of Human mTOR.

  • Western blot (WB) analysis of mTOR (S2442) at 1:500 dillution Lane1:NIH-3T3 whole cell lysate(40μg) Lane2:HEK293T whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg) Lane4:Hela whole cell lysate(40μg) Lane5:MCF-7 whole cell lysate(40μg)

  • Immunohistochemistry (IHC) analyzes of mTOR (S2442) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing endoplasmic reticulum membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

  • Western blot analysis of extracts from HEK293T cells, no-transfected(Lane 1), transfected with control shRNA (-) (Lane 2) or mTOR shRNA (+)(Lane 3). mTOR was detected using mTOR Antibody BS3611.The mTOR Antibody confirms silencing of mTOR expression.

Background

mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.CE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal Antibody


CE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal AntibodyCE, ISO9001 Approved Mtor (S2442) Polyclonal Antibody

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