Product Introduction

Toluidine Blue is a quinoneimine basic dye, which can combine with acidic substances in tissue cells to achieve tissue staining. The stained cell nucleus is blue; the Nissl body in the neuron can be stained blue by toluidine blue, which can be used for preliminary pathological diagnosis of Nissl body; the cytoplasm of mast cells contains heparin and histamine, and the cartilage tissue contains cartilage sulfate These substances have metachromatic properties and are metachromatic with toluidine blue and are purple-red. Therefore, toluidine blue staining can be used to observe the distribution and abnormal changes of mast cells, and observe the morphology and structure of cartilage such as tide lines. Plant tissues can be stained and observed. The xylem, ducts, sieve tubes and other structures, the xylem is blue-green, and the cellulose cell wall is blue-purple.

The active ingredient concentration of the toluidine blue dye solution of this product is 0.5%, which can be used for the dyeing of conventional animal and plant tissue sections.

Storage and transportation

Storage and transportation at room temperature, valid for 18 months.

Instructions

1. Dewax the paraffin sections to water;

2. Toluidine blue staining: the tissue sections are stained with toluidine blue staining solution for 2-5 minutes, and then washed with tap water to slightly wash off excess staining solution.

a. Plant tissues are sliced, washed with water and examined under microscope. According to the degree of staining, use 0.1% glacial acetic acid for proper differentiation. If the degree of coloring is appropriate and does not require differentiation, place the slices in an oven at 60°C for drying.

b. Animal tissue slices, washed with water and differentiated with 0.1% glacial acetic acid. Observe the degree of differentiation under a microscope, and differentiate until the background is light blue, Nissl body, cartilage, mast cells, etc. are clearly colored. The differentiation was terminated by washing with tap water, and the sections were dried in an oven at 60°C.

3. Transparent mounting: the slices are transparent with xylene for 10 minutes, and then the slides are sealed with neutral gum.

Note: Bring your own 0.1% glacial acetic acid, xylene, neutral gum, etc.


Precautions

1. After dyeing, the section should not be washed for too long, otherwise the color will fade easily.

2. The slices must be completely dried before transparent mounting to prevent the remaining small water droplets from affecting the observation.

3. Please wear lab coat and disposable gloves during operation.