Phalloidin Staining Reagent Red Light Immunofluorescence Analytic Reagent

China Phalloidin Staining Reagent Red Light Immunofluorescence Analytic Reagent, Find details about China Phalloidin with Fluorochrome, Staining Solution from Phalloidin Staining Reagent Red Light Immunofluorescence Analytic Reagent

Basic Info.

Model NO.

G1041-50UL

Feature

Fluorochrome Red

Package

Bottle

MOQ

100 Bottles

Storage Conditions

-20ºC

Storage

Sealing in Dark

Expiration Date

12 Months

Trademark

Servicebio

Transport Package

Standard Package

Specification

50ul/Bottle

Origin

China

HS Code

3204901000

Model NO.

G1041-50UL

Feature

Fluorochrome Red

Package

Bottle

MOQ

100 Bottles

Storage Conditions

-20ºC

Storage

Sealing in Dark

Expiration Date

12 Months

Trademark

Servicebio

Transport Package

Standard Package

Specification

50ul/Bottle

Origin

China

HS Code

3204901000

Product Desciption

50ul High Tech Phalloidin Stain Labeld by TRITC

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. As a result of binding phalloidin, actin filaments become strongly stabilized.
This product is a Phalloidin labeled with fluorescent dye TRITC. The emission wavelength of TRITC is 570-573 nm, which can be used for fluorescence detection of microfilaments in cells or tissues. The cytoskeleton shows red fluorescence after staining. The recommended dilution ratio for this product is 1:300-1:500. This product can be used for dyeing 70-120 slides according to the requirement of 200 μL dyeing solution per sheet.

Preservation:
Valid in 12 months when stored at -20ºC and kept in dark condition.

Procedure:

1.Cell slides or tissue sections fixed with formaldehyde (G1101 recommended), washed with PBS (G4202 recommended), dried slightly, and then circled with a histochemical stroke to place the tissue or cells in the center of the circle.
2.PBS containing 0.1% Triton X-100 or membrane breaking solution (G1204 is recommended) was dropped into the circles to completely cover the tissues. The tissues were incubated at room temperature for 10 min, and washed with PBS for 3 times, 5 min each time.
3.The product was diluted with PBS at the rate of 1:300 to 1:500 to prepare a working solution for Phalloidin Stain.The tissue was completely covered by dropping the staining solution of phalloidin stain into the circle. The tissue was stained at room temperature in dark for 60-120 min, and the sections were washed with PBS for 3 times.
4.(Optional) Drops of DAPI staining reagent (recommended G1012) were added to the tissues to stain the nuclei for 8-10 min, and the sections were washed with PBS for 3 times.
5.After the slices were slightly dried, anti-fluorescence quenching sealing tablets (G1401 is recommended) were added to seal the slices and observed under a fluorescence microscope or confocal laser microscope.

Phalloidin Staining Reagent Red Light Immunofluorescence Analytic Reagent

Notes:

1.Fluorescent dyes have the problem of quenching. Avoid light when operating.Dyeing solution is prepared and used as soon as possible and cannot be stored for a long time.
2.Phalloidin stain are generally not cellular permeable and are rarely used for staining of living cells.
3.Please wear a lab coat and disposable gloves when operating.