Product Introduction
In histopathology, the tissue must be fixed in time and effectively before the subsequent embedding and sectioning can be completed. The role of tissue fixation is to maintain the inherent morphology and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissue cells, preserve the inherent substances in cells, coagulate intracellular proteins, and minimize or terminate endogenous and exogenous The enzyme reaction keeps the cells or tissues basically the same as the substances in life, and can also harden the tissues and facilitate the cutting of the tissues. For some infectious specimens, it can prevent the spread of diseases; keep the general specimens. Finally, fixation can also enhance the effect of dyeing.
The FAA fixative (50%) of this product, namely formaldehyde-ethanol-glacial acetic acid fixative, is prepared from 5% formalin, 5% glacial acetic acid, and 90% 50% ethanol. It is suitable for the fixation and preservation of tender plant roots, stems, leaves, anthers, and ovary tissues, and is widely used in the study of plant morphology and anatomy. The biggest advantage of this fixative is that it has the function of a preservative, but it is not suitable for the observation of chromosomes.
Storage and transportation
Transport at room temperature; store in a cool and dry place, avoid direct sunlight, valid for 24 months.
Instructions
Remove the plant tissues and rinse them with water, and then put them in FAA fixative. Larger tissues need to be cut into small pieces and fixed. It is generally fixed in FAA fixative for more than 24 hours. Smaller or young tissues can be appropriately reduced in time according to the size of the material. If possible, the sample is immersed in the fixing solution, and then vacuum can be used to promote the fixation of the sample.
Precautions
1. Please use it in a well-ventilated environment, and take precautions to avoid inhalation.
2. In order to ensure that the fixative fully penetrates the tissue, vacuum treatment can be added.
3. Please tighten the cap in time after use to prevent the active ingredients from volatilizing.
4. For your safety and health, please wear lab coats and disposable gloves for operation.