Instructions for use

If you need to counter-stain cell nuclei, prepare your own hematoxylin staining solution, hematoxylin differentiation solution and hematoxylin returning blue solution. Bring your own ethanol, xylene, neutral gum, etc.

1. Paraffin sections are deparaffinized and rehydrated according to conventional procedures.

2. Congo red staining: the sections are soaked in Congo red staining solution overnight, and then washed with tap water for 2 min.

3. Differentiation: The slices are differentiated into positive amyloid plaques by alkaline differentiation solution, the background is basically colorless, and then washed with tap water.

4. (Optional) Staining nuclei: Stain the slices in hematoxylin staining solution (G1004) for 1 min, differentiate with hematoxylin differentiation solution (G1039), wash with tap water, return to blue with hematoxylin blue solution (G1040), and rinse with running water.

5. Dehydration and mounting: the slices are dehydrated three times with absolute ethanol for 5 minutes each time; the sections are transparent with xylene twice, each time for 5 minutes. Add neutral gum to seal the sheet.


Precautions

1. The alkaline differentiation solution needs to be sealed and stored to prevent the evaporation of the solvent from affecting the differentiation effect. Use it as soon as possible after opening.

2. Differentiation time varies according to slice thickness, tissue type, etc. After differentiation, wash with water in time to stop differentiation to prevent over-differentiation, and at the same time ensure sufficient washing to completely remove the differentiation solution.

3. Please wear lab coat and disposable gloves during operation.