Description

The PCR Kit contains a supply of recombinant, highly purified Taq DNA Polymerase, and a premixed, ready-to-ues 2x PCR Reaction Mix to perform 200 PCR reactions.

2x PCR Reaction Mix is a premixed, ready-to-use solution containing dNTPs and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, please mix the polymerase and the reaction buffer in appropriate proportion, and then add the primers and template DNA. PCR Kit contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Using the kit in your PCR reaction results in 3'-dA overhangs PCR products.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 % TW 20, 0.5 % NP 40, 50 % Glycerol

Composition of 2X PCR Reaction Mix

100 mM KCl, 20 mM Tris-Cl, 3 mM MgCl2, 400 μM dNTPs, bromophenol blue, 3d H2O

Features 

Efficiency: simplifying the operation and saving your time

Reproducible: reduce the risk of pipetting errors, miscalculation and contamination

Flexible: the amount of polymerase is flexible and controllable. 

Applications

• PCR amplification of DNA fragments as long as 5 kb

• DNA labeling.

• DNA sequencing.

• PCR for cloning.