Product Introduction
The full name of M199 is Medium 199, which is medium 199. It was originally designed for the cultivation of chicken embryo fibroblasts, and is currently widely used in the cultivation of various animal cells, including non-mammalian animal cells. Compared with other basic media, M199 contains some unique ingredients, including adenine, adenosine, hypoxanthine, thymine and other vitamins. Different balanced salt ingredients can be used. For example, Earle's salt is suitable for CO2 environment, while Hank's salt Suitable for non-CO2 environment. M199 is particularly suitable for the cultivation of non-transformed cells, and is also commonly used for the cultivation of virology, rat pancreatic epithelial cells and mouse lens tissue.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture. It does not contain protein, lipids or growth factors. Serum or serum-free additives should be added according to the needs of the cells.
This product is sterilized by filtration through a 0.1 μm filter membrane, pH 7.0-7.4, contains Earle's balanced salt system, contains 1000 mg/L D-glucose, and adds L-glutamine.
Storage and transportation
Transport at room temperature; store at 2-8°C, valid for 12 months.
Precautions
1. This product has been filtered and sterilized. When using it, you should pay attention to aseptic operation to avoid contamination.
2. In order to maintain the best results, please do not freeze-thaw.