Q: Can QPCR be used directly upon arrival? Does new machine need calibration after transporation ?  

A: It need to be unlock first when arrive . There is video instruction.  No need calibartion .

Q: What is the requirment of PC when install software ?

A: It need Win10 system PC. For new PC, it need to install a driver before install software. During the runing of software, PC screen need to keep on , not use dormant status.

Q: What is the difference between 4 channel and 6 channel model ?

A: PCR-RT-96-6 has six detection channels. PCR-RT-96-4 has five detection channels. They all have 2 duplicated for same dyes SYBR / FAM.  PCR-RT-96-4 have one channel extra - Cy3/NED/TAMRA

Q: What is the difference between Standard and Fast mode for Melting curve?

A: Both model QPCR have 2 FAM/SYBR channel. For SYBR , in melting curve  FAST Mode, the 2 FAM channel will be open at same time and detection time will be shorter than standard mode.  But FAST mode cannot be used in Taqman probe mode.

Q: Can we adjust the voltage(Gain value )of PMT Setting of different channel ?

A: It cannot adjust the voltage of each channel , but we can adjust the light path with 3levels accordng to customer requirement to meet the demand of different signal intensity.

Q: What is the Scanning method?

A: From the top to scan each tube in sequence. The advantage is - avoid the crosstalk and get accurate fluorescence value.

Q: What is the door direction ?

A: QPCR door  is at front side of machine.

Q: Does QPCR result  need to be corrected by ROX ?

A: Not need  

Q: What is the Detection device of QPCR? What light source  and how many?

A: The detection device is PMT.  The light source is LED. X6-6  X4-5

Q: What is the difference between PMT and CCD detection

A: PMT is  highly sensitive signal detector. The detector scans the fluorescence signal of each well separately.  It can effectively reduce fluorescent crosstalk. CCD is like a high-sensitivity camera.It collects fluorescence values of all well positions at once.It takes less time.

Q: What is the sample volume ?

A: 10-50ul

Q: What size tube can be used on QPCR ?

A: Recommend 100ul PCR tube or low profile 200ul tube.   If use 200ul high profile tube , it will appear steam in the tube which may affect the test result. If must use 200ul high profile tube,  suggest to add paraffin oil .

Q: It only has Second unit when we set the timer,   can we have option of Minute ?

A: The length of gene is usually 200bp,  time required  as Seconds level. Only in RT test ,  Minute is needed .  Will consider to add Minute unit in future.

Q: Can we edit program while QPCR run the program ?

A: Cannot edit .

Q: During PCR running , how to check the previous test result ?

A: Click 'File- Open in frame '

Q: What is the ordinate of the amplification curve currently showing?

A: In the latest version of the software, the original fluorescence value, Rn value and the normalized fluorescence value curve can be displayed.

Q: In the absolute quantitative test, can result analysis show the average value and deviation?

A: It need to tick out Replicate (in sample setting interface)  when set Replicate sample and standard sample as the same value , then it appears average value during analysis , but cannot show the deviation value.

Q: Can user delete those unnormal results whe make the standard curve ?

A: The software does not support  direct edit after test , user can output the CT value data and edit it in excel, then make the standard curve in excel.

Q: How to avoid the wrong setting or miss-selection of channels ?

A: Software doesnot support analysis after result, Suggest user to tick out all the channels during setting.

Q: How to adjust the threshold value ?

A: Select Spine method,  threshold line will appear  and can be adjusted

Q: What is the suitable value of Threshold line ?

A: For Linearity curve , put it at inflection point.  For logistic curve, put it at straight line place

Q: The application result is normal why data cannot output ? Why the aplication curve cannot display when tick out the reaction tubes position ?  

A: Check the Return at the right corner of Amplification interface,  click Return then can operate.

Q: Can user  define the color of amplification curve ?

A: CAN NOT

Q: How to adjust the start point of  baseline after test end ?

A: The baseline has been automatically corrected , not need to adjust . The latest version of the software provide the option of baseline adjustment.

Q: How to solve the issue that signal value reach to upper limit at platform stage?

A: Can adjust the concentration of  fluorescene marker or set the PMT detection as Low level .

Q: Can we use Molecular beacon to do melting curve ?

A: Hardware can support this test, but software doesnot have this analysis function, will be added in the next version software.

Q: Does QPCR have HRM analysis ?

A: The software is not supported currently

Q: What need to be attention when move the instrument ?

A: Before move the instrument , put the machine in 96 well protection plate and lock the machine.   Refer to the Lock operation video.